Reduction of four bis‐azo dyes by pig liver microsomal fraction in anaerobic conditions

The study of azo dyes degradation products and their harmfulness is an important topic because diffusion in the environment still a serious problem. We investigated reduction by pig liver microsomal fraction four bis-azo dyes, Acid Red 73, 150, Direct 24, 28, anaerobic condition. These complex molecules, different for position/nature substituent groups group interposed between two bonds, allowed to investigate correlation substrate molecular structure action subcellular representative xenobiotic living organism consequent possible appearance harmful reaction products. Dyes were first carefully purified eliminate interferences enzymatic assays. identified gas chromatography/mass spectrometry. 73 150 markedly reduced with production aromatic amines, while 24 28 neither synthesis amines nor other amines. results suggest that benzidine derivative are not degraded therefore molecules similar could be classified lower risk index producing any catabolic product.